Chromatography Forum

Dear Chromatography Users

please suggest any HPLC method conditions for Cyclophosphamide Related substances or any other techniques. For this compound USP method available for assay, but for Degradants given TLC method. In our HPLC development trails we observed all impurity peaks are eluting at void volume with diffrent columns and mobile phases. All impurities are observed very polar in nature. So please share your valuable experience. Thanks in advance

Kishore

Try this reference from ScienceDirect:

http://www.sciencedirect.com/science/ar ... 3209002876

Matt

Sorry, this one is much better:

http://www.sciencedirect.com/science/ar ... 479900300X

Matt

Dear MattM,

thank you for your reply. But i am facing problem with the analysis of cyclophosphamide impurities. Cyclophosphamide elution is about 5 min, but all A,B,C and D Impurities are eluting at void volume. All are non-chromophoric natured compounds and very polar in nature. Can u give any suggestion related to this kind of problem.

Regards
kishore

Hi Kishore,

Perhaps, in a general way. Please can you provide the separation conditions that you are trying to use, such as the column phase type, dimensions, eluent composition, eluent flow rate, sample solvent and intended mode of detection? These data may help provide a path forward toward better separation conditions to employ.

There are a variety of reversed-phase column stationary phases that are more appropriate for polar analytes than others, as well as HILIC, that may be brought to bear on your separation.

Please, see what you think, and my thanks.