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HPLC Sample preparation

http://www.chromforum.org/viewtopic.php?t=22526

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HPLC Sample preparation

Posted: Sat Apr 27, 2013 9:57 am
by faslu8415
HPLC sample preparation

Dear all

My mobile phase is ACN:H2O (70:30),Isocratic, and the sample to be injected is levenogestrel eluted into simulated uterine fluid (contains salts). When i am injecting the sample into the said mobile phase precipitate formation occurring , kindly suggest me a sample preparation method to overcome this...


Thanking you

Faslu Rahman
Central instrumentation laboratory
R&D Centre
HLL Lifecare Limited
Trivandrum

Re: HPLC Sample preparation

Posted: Sat Apr 27, 2013 4:14 pm
by tom jupille
Add an equal volume of acetonitrile to your sample, mix thoroughly, centrifuge, and inject some of the supernatant.
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