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ghost peak in GPC

http://www.chromforum.org/viewtopic.php?t=22479

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ghost peak in GPC

Posted: Mon Apr 22, 2013 5:51 am
by push
Hi,
I use anhydrous 1,2,4-trichlorobenzene in our high temperature GPC (120C), using BHT as an added antioxidant. I have noticed that the fresh solvent does not reveal any ghost peak, but after 6-7 injections of a blank sample (TCB+BHT) a ghost peak rises in LALS and RALS signals in low molecular weight region.
What can cause this peak?

Re: ghost peak in GPC

Posted: Tue Apr 23, 2013 1:01 pm
by Gerhard Kratz
How long is one run? Maybe this is a temperature problem. Are you sure that your mobile phase is at the same temperature than the column in the oven when entering the column?

Re: ghost peak in GPC

Posted: Tue Apr 23, 2013 2:28 pm
by tom jupille
The various oxidation products of BHT ?

http://195.37.231.82/publications/pac/p ... 0x1615.pdf
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