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flow rate gradient along with composition gradient

Posted: Tue Jul 19, 2005 5:39 pm
by Ericz
I'm trying to run a gradient LC method by setting both flow rate and mobile phases in gradient mode such that the thod can cover a variety of compounds. Any suggestion about the method? Thanks!

Posted: Wed Jul 20, 2005 2:01 am
by Uwe Neue
One thing to think about with variable flow rate methods is that your calibration will vary with retention.