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Chemstation number of signals

Posted: Wed Apr 10, 2013 5:47 am
by Leni
Hi,
the current version of Chemstation i'm using on HPLC's (DAD!) enables me to record up to 5 different signals at once(5 different wavelengths). The thing is, we are trying to allign our method of PV to the one used by Agilent during OQ/PQ, which for the Wavelength Accuracy test presumes that we record signals for at least 205nm +/- 3nm and 273 +/- 3nm (caffeine standard)...so basically at least 2x 7wavelengths (202...208nm and 270...276nm)...how could i add some signals, or maybe solve this issue otherwise.
Any suggestion would be appreciated,
Leni

Re: Chemstation number of signals

Posted: Wed Apr 10, 2013 11:30 am
by Csaba
We are recording the chromatogram at one wavelength only, BUT also recording the spectra.
Then at the chromatogram peak, we view the spectrum to verify wavelength accuracy by determining the wavelength position of the two peaks. Hope it help.

Re: Chemstation number of signals

Posted: Fri Apr 19, 2013 4:40 am
by Leni
We are recording the chromatogram at one wavelength only, BUT also recording the spectra.
Then at the chromatogram peak, we view the spectrum to verify wavelength accuracy by determining the wavelength position of the two peaks. Hope it help.
Kosziiiiiii...
Yes, this is also the solution i have found to be best...:) Thanks a lot!
Lenard