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Anion Column
Posted: Sat Mar 23, 2013 3:28 pm
by rtanco1
Hello, does anybody know how to re-generate an Anion /S column? Packing is Silica w/quaternary ammonium ion exchanger. Mobile phase is 4 mM KHP.
Thank you very much.
Raul
Re: Anion Column
Posted: Mon Mar 25, 2013 10:34 am
by Gerhard Kratz
Maybe the manual or handbook you got from the manufacturer of your column can help you to do a kind of regeneration of the column, but in most cases that will not work and it is time to order a new column. Sorry.
Re: Anion Column
Posted: Mon Mar 25, 2013 3:32 pm
by tom jupille
A higher concetration of KHP and / or a higher pH. But those columns are notoriously short-lived:six months or so in aqueous solution (even if you don't run any samples). Storing in methanol "stops the clock" on the deterioration.