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[reddyashwin]

Dear all.
what is use of pore size in column

regards,
ashiwn

[lmh]

If you work on small molecules it's of very little relevance, but if you work on large things, there can be a size-exclusion effect where the big thing cannot interact with the column particularly efficiently because it cannot diffuse in and out of the pores.

[chromatographer1]

In other words, many analytes that are similar in size do not separate well on a smaller pore size silica support.

Single broad peak peptides were found 35 years ago to separate into several sharp peaks when a column or 300A or larger pore size was used instead of a 60A.

Larger pore particles are not as durable as the 60A particles.

Take your pick.

Rod