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- Posts: 1
- Joined: Wed Mar 20, 2013 4:34 pm
The Totalchrom software will not recognize these bunch of peaks, and it is giving a diagonal integration line from the base of the first peak to the valley of the largest (oleamide) peak.
My question is, in Totalchrom, how would I set this (manually or by baseline event) to integrate these peaks such that I can get areas for all of these individual components... The only thing I've seen that works thus far is using the manual integration to integrate all the peaks as one, then approximate the peak area of the first, and then subtract that (times the number of non-analyte of interest peaks) out of the total grouped area (basically, playing the guessing game - not good science).
Any help here would be greatly appreciated.. I've read through the pdfs and tried calling tech support - the only advise i've received so far is to go to their training class, which doesn't solve the issue of my boss wanting results ASAP.
PS, I can take a screenshot and email it if you want a visual to help
Thanks!
