Chromatography Forum

Dear all.
what is use of pore size in column

regards,
ashiwn

If you work on small molecules it's of very little relevance, but if you work on large things, there can be a size-exclusion effect where the big thing cannot interact with the column particularly efficiently because it cannot diffuse in and out of the pores.

In other words, many analytes that are similar in size do not separate well on a smaller pore size silica support.

Single broad peak peptides were found 35 years ago to separate into several sharp peaks when a column or 300A or larger pore size was used instead of a 60A.

Larger pore particles are not as durable as the 60A particles.

Take your pick.

Rod