Chromatography Forum

I have a set of peaks (isomers of oleamide probably) that are eluting very close together. Using Peaksimple in the past, I would have simply used the peak split function b/t the bunched peaks which would drop down to the baseline and give separate areas for the peaks.
The Totalchrom software will not recognize these bunch of peaks, and it is giving a diagonal integration line from the base of the first peak to the valley of the largest (oleamide) peak.
My question is, in Totalchrom, how would I set this (manually or by baseline event) to integrate these peaks such that I can get areas for all of these individual components... The only thing I've seen that works thus far is using the manual integration to integrate all the peaks as one, then approximate the peak area of the first, and then subtract that (times the number of non-analyte of interest peaks) out of the total grouped area (basically, playing the guessing game - not good science).
Any help here would be greatly appreciated.. I've read through the pdfs and tried calling tech support - the only advise i've received so far is to go to their training class, which doesn't solve the issue of my boss wanting results ASAP.

PS, I can take a screenshot and email it if you want a visual to help
Thanks!

You may want to try using the Horizontal Baseline On (+HF) and then Start New Peak (S) for each individual peak. You also will probably have to to play with the Bunching Factor, Noise and Area Threshold values. You also might want to use Horizontal Baseline off (-HF) if you do not want a forced baseline across your entire chromatogram.