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Additives such as Tween 80, Cyclodextrin and pluronics
Posted: Fri Mar 08, 2013 2:38 pm
by ivarg
Hi
Does anyone have any experience with the above mentioned as additives in samples for HPLC (what conc. is recomended)? Columns used are biphenyl 3µm 100X2,1 and C18 5µm 100X2,1. Mobilfase consist of methanol and water with salt pH around 7,5.
Thanks for the help.
Ivar
Re: Additives such as Tween 80, Cyclodextrin and pluronics
Posted: Wed Mar 13, 2013 2:30 pm
by danko
Why would you like to add these to the sample solutions?
Best Regards
Re: Additives such as Tween 80, Cyclodextrin and pluronics
Posted: Thu Mar 14, 2013 9:50 am
by ivarg
I'm not the one that want to add, I just get to analyze.... Someone one time mentioned something about amount in sample for LC, but I can't remember where, when or what. They use those 3 to solubilize organic molecules that are not soluble in water. Then they would like a nice peak from HPLC.
Best regards
Ivar
Re: Additives such as Tween 80, Cyclodextrin and pluronics
Posted: Thu Mar 14, 2013 10:25 am
by danko
Hi Ivarg,
Well the one that wants these additives in the sample solvent might be forgetting that the molecules to be analyzed need to be soluble in the mobile phase as well – even more so. Otherwise they’ll get stuck on the whole system including the column and you’ll see no “nice peaks”
Best Regards