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GCMS result interpretation
Posted: Wed Mar 06, 2013 4:53 pm
by hansrajh
my friend have a GCMS result.. but we dont know how to use it.. that file contain three library hit compounds.. how can i find which one is our compound?
Re: GCMS result interpretation
Posted: Wed Mar 06, 2013 9:21 pm
by Steve Reimer
The usual way is to compare the retention time with standards of the possible compounds. If you don't have standards of any of them you may be able to eliminate choices based on the retention time of your unknown and known compounds eluting close to it in time.
In some cases you may not be able to determine which, if any, of the library matches is correct without a standard.
Re: GCMS result interpretation
Posted: Thu Mar 07, 2013 4:22 am
by Don_Hilton
And, sometimes no library match hit is correct.
The easiest path for identification of an unknown is knoledge of your sample. This suggests things that might be present.
Re: GCMS result interpretation
Posted: Sat Mar 09, 2013 1:54 am
by MSCHemist
sometimes that can be a problem. Especially the terpenes I can get muliple high match hits. I had a chromatogram last week that had alpha pinene in 4 different places according to the library report. Alkanes can be tricky too as well as some isomers.