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why caffiene and acetone used in HPLC calibration

http://www.chromforum.org/viewtopic.php?t=22027

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why caffiene and acetone used in HPLC calibration

Posted: Thu Feb 28, 2013 4:16 pm
by reddyashwin
Dear all,
why all are using the caffiene and acetone in HPLC calibration.In HPLC calibration methyl,ethyl,proply parabene using why explain.


regards,
ashwin

Re: why caffiene and acetone used in HPLC calibration

Posted: Fri Mar 01, 2013 12:22 am
by tom jupille
They are available, cheap, and pure; are easily detectable via UV absorbance, and generally "well behaved" neutral compounds.
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