RP Column Stability Under Reducing Conditions
Posted: Wed Feb 20, 2013 8:51 pm
I'm trying to analyze some compounds that must be reduced (and stay reduced) to be water soluble by UPLC. I am adapting a method our lab previously used with CE where we included 20 mM thiourea dioxide (Eo ~ -1000 mV, yikes) in the mobile phase buffer at pH 9.3.
My question is whether or not thiourea dioxide (or any other comparably strong reducing agent) is going to damage my C18 column. It is a Waters Acquity BEH C18 UPLC column. I've asked Waters and they didn't have an answer. Does anyone have any experience doing RP LC under strong reducing conditions? The rest of the method is typical, involving MeOH/ACN, ammonium acetate buffer, column at 25 C / 40 C, etc.
Thanks a bunch!
My question is whether or not thiourea dioxide (or any other comparably strong reducing agent) is going to damage my C18 column. It is a Waters Acquity BEH C18 UPLC column. I've asked Waters and they didn't have an answer. Does anyone have any experience doing RP LC under strong reducing conditions? The rest of the method is typical, involving MeOH/ACN, ammonium acetate buffer, column at 25 C / 40 C, etc.
Thanks a bunch!