peak width software help (xcalibur, and totalchrom)
Posted: Mon Feb 18, 2013 3:18 am
Hi all, I am sorry to pop on here and first thing ask, but I've searched somewhat extensively on the web and on this forum. I'm a lab tech at a small university and I am testing an experiment they want to try (just separation of some different benzenes in meoh/h2o).
I am using a Thermoscientific Finnigan LC (coupled with MS, but have MS off). The software is xcalibur. I can't figure out how to do peak width at 50%. There is a very brief explanation of peak width in the help, but no reference as to how to view it (I'm not even sure it's possible, although it seems it should be). I can export the chromatogram to CSV and open it in excel, and was hoping to find a simple tool that undergrad students could use to find the peak width @50, but I can't find that either. Ideally, I'd like a free software you can import a CSV chromatragram into, but I don't think those exist.
Secondly, and less importantly. I have a way to get peak width at 50% for our TotalChrom (perkin elmer) on the other LC we're using for the class, but it's a lengthy and annoying process for each chromatogram, which involves running everything, using graphic edit to re-identify components, running system suitability on all of them, then opening the report for each.
I've been working all weekend on the experiment, and unfortunately it's tomorrow and I still don't have a way to get peak width on the xcalibur. I'm hoping I don't get an evil eye if I tell them to calculate it manually with a ruler on the screen.
Very grateful for any help,
Thanks !
I am using a Thermoscientific Finnigan LC (coupled with MS, but have MS off). The software is xcalibur. I can't figure out how to do peak width at 50%. There is a very brief explanation of peak width in the help, but no reference as to how to view it (I'm not even sure it's possible, although it seems it should be). I can export the chromatogram to CSV and open it in excel, and was hoping to find a simple tool that undergrad students could use to find the peak width @50, but I can't find that either. Ideally, I'd like a free software you can import a CSV chromatragram into, but I don't think those exist.
Secondly, and less importantly. I have a way to get peak width at 50% for our TotalChrom (perkin elmer) on the other LC we're using for the class, but it's a lengthy and annoying process for each chromatogram, which involves running everything, using graphic edit to re-identify components, running system suitability on all of them, then opening the report for each.
I've been working all weekend on the experiment, and unfortunately it's tomorrow and I still don't have a way to get peak width on the xcalibur. I'm hoping I don't get an evil eye if I tell them to calculate it manually with a ruler on the screen.
Very grateful for any help,
Thanks !
