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Sennosides
Posted: Thu Jul 07, 2005 1:43 pm
by Fernando
Does anyone knows of a HPLC method for the determination of Sennosides A & B?
I¨ve been using the BP colorimetric method.
Thanks to all.
Re: Sennosides
Posted: Fri Jul 08, 2005 6:35 am
by poetsch
Dear Fernando,
you may have a look at the following publication, which still can serve as a basis for further analytical development (although it is ten years old):
Kabelitz; Reif. Deutsche Apotheker Zeitung 134 No. 51/52, pp. 5080 (1994)
Best regards
Sennosides
Posted: Fri Jul 08, 2005 7:46 am
by michaelcarolus
Hi Fernando
Try the following article: Validated HPLC method for the determination of sennosides A and B in senna tablets.
Shao-Wen Sun, Hsiu-Ting Su, Journal of Pharmaceutical and Biomedical Analysis 29 (2002) pg 881 - 894.
I hope this helps
Cheers
Mike
Sennosides
Posted: Fri Jul 08, 2005 11:46 am
by Fernando
Hi poetsch and michaelcarolus
Thank you for the references, have any of you tried these methods?
Best regards.
Sennosides
Posted: Mon Jul 11, 2005 7:30 am
by michaelcarolus
Hi Fernando
We had success with the method for tablet formulations but the method was not suitable for our liquid formulations.
Good luck with your development.
mike
Sennosides
Posted: Mon Jul 11, 2005 7:40 am
by poetsch
Hi Fernando,
we used a slightly adjusted method for a herbal tea preparation with Senna leves and fruits as API. We had to adjust because the original method had its focus on selectivity in order to identify nearly all sennosides present in Folia Sennae. So we shortened the method for the assay, but it worked fine concerning the basic method parameters.
Good luck...
Sennosides
Posted: Wed Jul 13, 2005 2:41 pm
by Fernando
To michaelcarolus & poetsch
Thank you for the info
I´m searching for the articles.