Carry Over??

[amishshah]

Do control carry over allows in assay analysis?

[JMcK]

Generally you want to get all the analytes off in single run. If you're having carryover it could coelute with your analyte of interest. I don't know how much freedom you have to adjust the method. If allowed, you can extend the run to ensure all the analytes elute, or, if it's a gradient reverse phase method, increase the organic phase concentration at the end of the run to push off the remaining analytes. You may have to equilibrate longer after each injection. Just some thoughts.

[zheyin]

Generally you want to get all the analytes off in single run. If you're having carryover it could coelute with your analyte of interest. I don't know how much freedom you have to adjust the method. If allowed, you can extend the run to ensure all the analytes elute, or, if it's a gradient reverse phase method, increase the organic phase concentration at the end of the run to push off the remaining analytes. You may have to equilibrate longer after each injection. Just some thoughts.

These are not carryover.

[zheyin]

Do control carry over allows in assay analysis?

I'd allow < LOD detection in carryover tolerable in my SOP.

[JMcK]

Do control carry over allows in assay analysis?

These are not carryover.

I'd allow < LOD detection in carryover tolerable in my SOP.

I'm not sure what you're talking about then, and I doubt I'm the only one.