How to Get Chemstation to Display % M+1, M+2 for ion peaks?
Posted: Wed Jan 30, 2013 11:32 pm
by Incitor
I've been running reverse phase separations on an 1100 HPLC with G1946D detector and Chemstation v. B.04.03.
I haven't figured out yet how to get the percentage of M+1 and M+2 peaks for a given ion peak. Does anyone know how to do this? Does chemstation have the ability to do this?
Re: How to Get Chemstation to Display % M+1, M+2 for ion pea
Posted: Thu Jan 31, 2013 3:38 pm
by lmh
I'd extract the ion chromatograms, add them to the method in the signal details, and then specify the base peak, +1 and +2 isotope peaks as separate compounds. Since they'll coelute exactly, Chemstation will attempt to add these as three signals relating to the same chemical (i.e. in the table, they'll all have a single # number). You'll have to enter the peaks manually as new compounds, in the same way as you would for an exactly coeluting internal standard. Once you've got the three peaks as separate compounds, you can analyse and tabulate your data as normal, get the whole thing into Excel (batch processing) and then do the percentage calculation there.