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Fatty acid analysis

http://www.chromforum.org/viewtopic.php?t=2177

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Fatty acid analysis

Posted: Wed Jul 06, 2005 3:28 pm
by GT
Dear all,
I have to analyze a mixture of stearic and palmitic acid to determine the relative composition of the mixture. I can't use a GC method so can I make this with HPLC plus DAD detector ?
Any kind of suggestion will be appreciate.
Thanks
GT

Posted: Wed Jul 06, 2005 3:41 pm
by Consumer Products Guy
We usually use GC for this, as methyl esters. For HPLC we have used 4-phenylacetophenone derivatives: see Analytical Chemistry, Vol. 47, No. 14, pp. 2437-2439 (Dec. 1975).

Posted: Wed Jul 06, 2005 3:51 pm
by Daren
you can separate and detect stearic and palmitic acid with RP and ELSD detection. You won't see much signal with UV.
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