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HPLC without extraction step ?
Posted: Mon Jan 21, 2013 9:38 am
by KJO35
Hi,
Is it possible to perform HPLC based on a cell lysate without extraction step ?
Thanks
Re: HPLC without extraction step ?
Posted: Mon Jan 21, 2013 10:37 am
by Johnny Rod
What are the analytes? Lysate will be a mixture of all sorts of rubbish, at least use a guard column, but I would expect extraction is abetter way to go to protect your instrumentation, as a generalism
Re: HPLC without extraction step ?
Posted: Mon Jan 21, 2013 11:03 am
by KJO35
I would like to quantify intracellular Talinolol (β1-selective beta blocker) in Caco-2 cell line. And I use a guard column.