HPLC without extraction step ?

[KJO35]

Hi,

Is it possible to perform HPLC based on a cell lysate without extraction step ?

Thanks

[Johnny Rod]

What are the analytes? Lysate will be a mixture of all sorts of rubbish, at least use a guard column, but I would expect extraction is abetter way to go to protect your instrumentation, as a generalism

[KJO35]

I would like to quantify intracellular Talinolol (β1-selective beta blocker) in Caco-2 cell line. And I use a guard column.