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Mobile phase and Sample Injection

http://www.chromforum.org/viewtopic.php?t=21732

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Mobile phase and Sample Injection

Posted: Sat Jan 19, 2013 8:43 pm
by montana
Hello,
Analysis of creatinine HPLC-DAD Plasma.

Can i inject a sample treated with ACN directly in HPLC , with mobile phase phosphate buffer pH 7, 1% MeOH.
Can i change the composition of the mobile phase, in way to replace the 1% MeOH by 1%ACN.

Thank you

Re: Mobile phase and Sample Injection

Posted: Sun Jan 20, 2013 3:38 pm
by Consumer Products Guy
When injecting a sample in strong organic solvent into a mostly-aqueous mobile phase, keep the sample injection volume SMALL.

Re: Mobile phase and Sample Injection

Posted: Sun Jan 20, 2013 6:45 pm
by montana
Thank you :D
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