What's the fastest way by analyzing omega-3 and omega-6
Posted: Thu Jan 17, 2013 8:34 pm
by mrpj
What's the fastest application of analyzing omega-3 and omega-6 from biological samples?
GC-MS or GC-FID ?
Re: What's the fastest way by analyzing omega-3 and omega-6
Posted: Thu Jan 17, 2013 8:38 pm
by JGK
What's the fastest application of analyzing omega-3 and omega-6 from biological samples?
GC-MS or GC-FID ?
Most official Methods such as AOCS, AOAC use GC-FID methods.
Re: What's the fastest way by analyzing omega-3 and omega-6
Posted: Thu Jan 17, 2013 11:45 pm
by mrpj
Ah okay, I found this one:
http://www.restek.com/pdfs/59584B.pdf when searching after "AOCS fatty acids".
Would there be any significant difference of using helium instead of hydrogen ?
Re: What's the fastest way by analyzing omega-3 and omega-6
Posted: Fri Jan 18, 2013 2:37 pm
by gserrano_84
There would be no difference. Chromatographers are inclined towards hydrogen when looking for faster analysis time (H2 allows you to keep a good column efficiency even at relatively fast linear velocities). When using He, your optimal linear velocity is lower than that of H2, if you wanna keep a similar column efficiency.
As it was previously said, most official methods use GC-FID, as you don't really need GC-MS for FAMEs.
Also if you are looking for cis/trans FAMEs, there is no 'fast analysis', you'll have to use longer columns (100-m), if identifiying cis/trans is not important, you can use 15-m 0.10 ID columns (and hydrogen) for faster analysis time and good separations.
Gustavo
Supelco