Chromatography Forum

If the injector of GC/MS is connect to the pyroyzer (a kind of reactor), how to do the quantitative analysis?

The Pyrolyzer-GC/MS system works as: the pyrolyzer will break the sample at very high temperature, and then the products will evaporate and go the GC/MS system.

But now I have problems to quantify the products. Cause the injector of GC is connected to the pyrolyzer through the transfer line. So if I want to make the calibration curve, I cannot inject the standards directly through the injector.

I know one option of the solution is to remove the pyrolyzer, so that I can do the injection. But I'm looking for another solution without remove the pyrolyzer, how to make the calibration curve.

Thanks and appreciate your help!

Perhaps, you can find a way to place the standard compunds into the pyrolizer and heat only enough to volatilize the standard compunds - and transfer them to the inlet. This may involve placing a solution of the compounds onto some inert material and placing that into the pyrolizer. You will have to do some checking to be sure you have complete (or maximum) transfer to the GC. If there is a loss of compounds at a higher temperature, you face the problem that you may have issues of loss even as you form the compunds - and quantification becomes an issue of measuring what you can...

I use a pyrolyzer system as well. What works best for us in our approach (polymers) is to spike your standards into a similar polymer matrix. Your pyrolyzer system may be different than ours. We have "sample cups" and a micro furnace. So the other possibility is to spike your sample into the sample cup and let the solvent evaporate (given that your spiked compound isn't volatile). Quantification is pretty easy and very straight forward after that.

I use a pyrolyzer system as well. What works best for us in our approach (polymers) is to spike your standards into a similar polymer matrix. Your pyrolyzer system may be different than ours. We have "sample cups" and a micro furnace. So the other possibility is to spike your sample into the sample cup and let the solvent evaporate (given that your spiked compound isn't volatile). Quantification is pretty easy and very straight forward after that.

My pyrolyzer system is CDS 5150. I think what you mean for sample cups is a kind of trap, which can adsorbed the pyrolysis products, is it correct?

No, I use the Frontier 3030d. It is a vertical microfurnace which uses 50 or 80 uL sample cups. It's a completely different system that the filament technology used by CDS.

I use a pyrolyzer system as well. What works best for us in our approach (polymers) is to spike your standards into a similar polymer matrix. Your pyrolyzer system may be different than ours. We have "sample cups" and a micro furnace. So the other possibility is to spike your sample into the sample cup and let the solvent evaporate (given that your spiked compound isn't volatile). Quantification is pretty easy and very straight forward after that.

My pyrolyzer system is CDS 5150. I think what you mean for sample cups is a kind of trap, which can adsorbed the pyrolysis products, is it correct?

I have systems both by CDS and FrontierLab. In case of the Frontier lab pyrolyzer, the sample cups can hold liquids, and hence you could easily load your standard and let it get injected onto the GC column. As for the CDS5150 (I still haven't run it - except for some nylon). I think we could soak some ) quartz wool in the standard solution (known quantity) and heat the coil up to about 200 deg C. This is just an idea, I'm hoping someone has a better one.

We have pyrolizer CDS 5150 too (connect to GC-MS), but we are new...I think can we make the standard curve by burn standard sample use the coil in the different weight?

Thx
Ito