Phosphate buffer

[thohry]

Hi all,

We have a method that uses Na2HPO4 as a buffer reagent. But the sodium salt is not available. Is it possible to use K2HPO4 instead.
Thanks

[tom jupille]

As far as pH control goes, it's the phosphate that's doing the buffering so the counterion shouldn't matter. You should check for solubility issues with other components of the mobile phase, though (potassium dodecyl sulfate, for example, is almost insoluble in water).

[thohry]

As far as pH control goes, it's the phosphate that's doing the buffering so the counterion shouldn't matter. You should check for solubility issues with other components of the mobile phase, though (potassium dodecyl sulfate, for example, is almost insoluble in water).

Thanks for your help.