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Improving Detection Limits
Posted: Wed Dec 19, 2012 2:52 pm
by sra37
I'm quite new to this, so bear with me. I am wondering if any of you have any tips for improving the detection limit of HPLC coupled with UV-Vis? So far I can't detect as low as I would like.
Is there anything I can do to improve this? (eg instrument settings, elution solvents, etc)
Re: Improving Detection Limits
Posted: Wed Dec 19, 2012 3:19 pm
by prepcolumn
at first glance you may; increase concentration, increase injection volume..
Re: Improving Detection Limits
Posted: Wed Dec 19, 2012 3:46 pm
by Consumer Products Guy
Better wavelength, solvents with less background absorbance, using gradient elution to sharpen peaks, actually diluting samples with water so a larger quantity can be injected without hurting peak shapes (I forget the term for this).
Re: Improving Detection Limits
Posted: Thu Dec 20, 2012 4:01 am
by DR
Don't forget that sometimes a narrower bore column can also help - eg: going from 4.6mm to 3mm.
Re: Improving Detection Limits
Posted: Thu Dec 20, 2012 9:48 am
by Johnny Rod
Anything that speeds up chormatography to turn wider peaks into taller peaks e.g. column temperature. Provided youre peaks are sufficiently resolved, you don't need glacial valleys between them
Re: Improving Detection Limits
Posted: Thu Dec 20, 2012 10:48 am
by rrm1987
look for some derivitisation agents which will enhance the UV-VIS absorbance.For which compound you would like to improve the sensitivity
Re: Improving Detection Limits
Posted: Thu Dec 20, 2012 9:55 pm
by sra37
Thanks for the suggestions everyone, I will have a play around with some of these ideas and see if anything works.