gradient sloping downwards
Posted: Tue Dec 18, 2012 4:23 pm
Hi and thanks in advance for helping out.
I am using acetic acid adjusted to pH 5 with ammonium hydroxide on A and 100% MeOH on B and I am monitoring at 230nm using a phenyl column. My problem is that the baseline slopes downward and I would like to correct for it and don't know how, any help would be appreciated.
I am using acetic acid adjusted to pH 5 with ammonium hydroxide on A and 100% MeOH on B and I am monitoring at 230nm using a phenyl column. My problem is that the baseline slopes downward and I would like to correct for it and don't know how, any help would be appreciated.