Extraction of (-)- Epicatechin from cacao
Posted: Wed Dec 12, 2012 7:30 am
by kez40
I am a student preparing a method to extract (-)- Epicatechin. How do I ensure results on the chrootogram will differentiate (-)-Epicatechin from (+) Epicatechin, stereoisomers that both elite at 280nm
Re: Extraction of (-)- Epicatechin from cacao
Posted: Fri Dec 14, 2012 1:43 am
by tom jupille
You will have to use an enantioselective ("chiral") column and optimize the conditions to separate your isomers. Seefor example:
http://www.ncbi.nlm.nih.gov/pubmed/22649938
Re: Extraction of (-)- Epicatechin from cacao
Posted: Tue Dec 18, 2012 6:56 am
by kez40
Thank you that article was very helpful