Chromatography Forum

Hi everyone!
I'm new in this forum but i'm so interested in and i have a question for you!

right now i'm to try the method to study 5ALA in laboratory (in aqueous solution or industrial formulations)
but it is so difficult to find 5ALA because is small and it have an amino acidic structure.
i need to find HPLC method that give me separation and quantitative determination. (but easy to use)

we can't try derivatization (as literature) because this method destroy molecules at high temperature.
we would use simple derivatization.
i have heard news about AccQ Tag kit from Waters. anybody know that???
how much cost???

i'm really happy if somebody wants to help me to find a method or everything about
thankkkksssss

SALA is a village in Latvia.

What are you doing?

Rod

Sorry about the previous post.

Ok, my eyes are tricking me.

You meant Ala-ala-ala-ala-ala, right?

Your question is highly dependent upon the matrix in which you seek to measure Ala-Ala-Ala-Ala-Ala

Preliminarily, I would look at TLC to evaluate your matrix, both normal phase and reverse phase. Cheap enough to do at the beginning.

You should have a RI detector for HPLC or some other means (post - deriv) to detect the peptide.

TLC should detect 0.1 microgram of 5X-ala per volume of solution spotted.

You might get lucky.

Rod

oh thank you chromatographer1,
but 5ALA means 5 aminolevulinic acid.
this molecule has an amino acid structure and it's very very small and instable.
it is so hard to find with chromatogram method.
i need a help!

i post a link about
http://en.wikipedia.org/wiki/Aminolevulinic_acid

thank you everybody!!!

Actually, since it is similar to an amino acid much of my comments might be applicable to δ-ala

Ninhydrin would be the detection spray and a simple TLC might be the best starting place. Any HPLC method on RP for an amino acid could be serviceable to your application.

Good luck,

Rod