Extraction of (-)- Epicatechin from cacao

[kez40]

I am a student preparing a method to extract (-)- Epicatechin. How do I ensure results on the chrootogram will differentiate (-)-Epicatechin from (+) Epicatechin, stereoisomers that both elite at 280nm

[tom jupille]

You will have to use an enantioselective ("chiral") column and optimize the conditions to separate your isomers. Seefor example:
http://www.ncbi.nlm.nih.gov/pubmed/22649938

[kez40]

Thank you that article was very helpful