Hi Everyone,

I am in process of developing HPLC method for combination of assay and impurities for naloxone. But from literature i got method parameters only for assay, but not for impurities.

I don't have much experience in developing method. can any one give me some suggestions in developing method.

Here are following chemical names of which i am interested:
10α-Hydroxynaloxone
Noroxymorphone
10β-Hydroxynaloxone
Naloxone
7,8-Didehydronaloxone
2,2´-Binaloxone
3-O-Allylnaloxone

Can anyone please help me

Thank you