Page 1 of 1
Kinetex Column Method Development
Posted: Wed Nov 28, 2012 6:31 pm
by CLSgroup
We recently purchased a Kinetex (core shell technology) C18 column made by Phenomenex and have been doing some method development. I was wondering if anyone has done any method development for carbonyls or explosives using this column and would be willing to share what works and what doesnt work. Specifically we have Kinetex 5u C18 100A New Column 250 x 4.6 mm.
Re: Kinetex Column Method Development
Posted: Sat Dec 01, 2012 1:30 am
by carls
Re: Kinetex Column Method Development
Posted: Sat Dec 01, 2012 1:46 pm
by Consumer Products Guy
Specifically we have Kinetex 5u C18 100A New Column 250 x 4.6 mm.
New particle technology in old-column hardware and particle size dimensions....just wondering why...
Re: Kinetex Column Method Development
Posted: Sat Dec 01, 2012 5:34 pm
by carls
The Kinetex 5um media provides the same performance benefits over fully porous media as its smaller counter parts (i.e. 1.3um, 1.7um and 2.6um).
The Kinetex 5um media provides a simple way to improve the performance of existing 5um methods. Since Kinetex 5um gives ~2x the efficiency of fully porous 5um media, methods where resolution or sensitivity are problematic can be improved with this simple like for like switch.
Re: Kinetex Column Method Development
Posted: Sun Dec 02, 2012 3:02 pm
by Consumer Products Guy
Oh yes: trying to keep dimensions, etc., consistent with the beloved USP....
Re: Kinetex Column Method Development
Posted: Mon Dec 03, 2012 10:49 am
by upamaniah
USP never say anything about your own method development, in fact they welcome the changes, if we are able to provide the documents as per their standard policies. May be FDA could have some regulatory requirement, if any, and all one need to have, justification, equivalence report, full method validation document, and convincing capabilities any internal and external agencies.
Re: Kinetex Column Method Development
Posted: Mon Dec 03, 2012 3:06 pm
by carls
The above statement applies to existing methods.
Re: Kinetex Column Method Development
Posted: Wed Dec 05, 2012 12:26 am
by CLSgroup
Specifically we have Kinetex 5u C18 100A New Column 250 x 4.6 mm.
New particle technology in old-column hardware and particle size dimensions....just wondering why...
The reason that we got that is due to the fact that we weren't happy with the separation with our current column. If we were we probably would have gone with a shorter column. So far we have good separation with the Kinetex column but are having issues with the sensitivity at our lowest detection limit. We currently are trying to find a happy medium between detection and sensitivity.