Phosphate buffer
Posted: Tue Nov 27, 2012 3:44 pm
by thohry
Hi all,
We have a method that uses Na2HPO4 as a buffer reagent. But the sodium salt is not available. Is it possible to use K2HPO4 instead.
Thanks
Re: Phosphate buffer
Posted: Wed Nov 28, 2012 4:48 am
by tom jupille
As far as pH control goes, it's the phosphate that's doing the buffering so the counterion shouldn't matter. You should check for solubility issues with other components of the mobile phase, though (potassium dodecyl sulfate, for example, is almost insoluble in water).
Re: Phosphate buffer
Posted: Wed Dec 05, 2012 3:45 am
by thohry
As far as pH control goes, it's the phosphate that's doing the buffering so the counterion shouldn't matter. You should check for solubility issues with other components of the mobile phase, though (potassium dodecyl sulfate, for example, is almost insoluble in water).
Thanks for your help.