Fortification samples

[tere_p]

Hello everyone,

I am new in this field and I don't know how fortification is used. I know it is useful when doing recovery studies in quantitaion analyses. I would apreciate if someone of you can help me.

Thanks in advanced.

[Consumer Products Guy]

For fortification or spiked samples, have placebo product made for you. Then add (weigh, or more typically by pipet) the active or sought-for substance at desired active levels, typically 80, 100, and 120% of target, do at least three times at each spiked level, and assay. That's "Accuracy".

Assay real-life product at least five times, that's "Precision". Second operator's repeat of the precision study is "Intermediate Precision".