Chromatography Forum

Hi,
i could really use some help with some problems i've encountered while working on Chemstation (Rev. B.03.02 [341]). So, the thing is, my reviewer asked me (beside others) to solve two things, and after a few days of trying, i still can't figure it out:

1) When i extract the spectra for a peak, the value of the maxima(s) should appear marked(marker + value) on the spectra on the final print/report. Besides from marking the maxima manually(with annotations), i couldn't figure out how to make Chemstation do that for me...and of course marking it manually didn't cut it any ideas how it could be done automatically?!

2) When integrating a chromatogram having to use "Split peak" method (interfering little peak on side of the peak of interest ), i have to calculate the ratio between the height from the drawn base of the peaks, until the point where the two peaks are joined, and the hight of the peak of interest. Problem is, i couldn't figure out, how to have that height calculated and appear on the report i was forced to measure it on paper and stuff....isn't there a way to find out the height from the baseline to the point where the two peaks are joined?!

Any help will be highly appreciated. Thanks in advance!!

Lenard

PS.here are links to pictures if you need help in understanding the problems i'm facing, to better emphasize.

http://i46.tinypic.com/2r2xa8l.jpg

http://i48.tinypic.com/1zexj69.jpg

No one?! Someone?! ...Anyone???

doesn't it show up when you 'print report' to screen?

doesn't it show up when you 'print report' to screen?

well no.
or at least, i couldn't find a type of report to make it appear.any ideas?

This should help you to get the values that you want to see.

1. Put the ChemStation into the Data Analysis view.

2. Load the signal that you wish to look at.

3. Choose the signal view.

4. Click on the 'Display each in the Same Scale' button. This has the downward pointing arrow.

5. Make sure that you are using the arrow image for the cursor.

6. Move the cursor so that the point is on the base line.

7. Right click the mouse. The cursor should change to a small black arrow.

8. Use the mouse the move this arrow along the chromatogram, it will follow the peaks. At the bottom of the screen you will the time and signal value for the postion of the arrow on the chromatogram.

9. To return to the normal arrow, just right click again

Hope this helps.

Gasman

This should help you to get the values that you want to see.

1. Put the ChemStation into the Data Analysis view.

2. Load the signal that you wish to look at.

3. Choose the signal view.

4. Click on the 'Display each in the Same Scale' button. This has the downward pointing arrow.

5. Make sure that you are using the arrow image for the cursor.

6. Move the cursor so that the point is on the base line.

7. Right click the mouse. The cursor should change to a small black arrow.

8. Use the mouse the move this arrow along the chromatogram, it will follow the peaks. At the bottom of the screen you will the time and signal value for the postion of the arrow on the chromatogram.

9. To return to the normal arrow, just right click again

Hope this helps.

Gasman

Thank you. From what i see you described how to see the absorbance on certain points of the baseline, but that's not the problem i am facing. I don't know how to make maxima be noted on it automatically on a report (see one of the pictures for details)

Anyone?!?!?! No...?!?