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Rising curve at the end of chromatogram
Posted: Mon Nov 19, 2012 2:31 am
by ito_miura
Hi all.
I have a problem where the curve at the end of the chromatogram is rising instead of flat, near the base peaks. Is this a normal occurence? Otherwise, why could possibly make the chromatogram spike up?
Thanks in advance.
Re: Rising curve at the end of chromatogram
Posted: Mon Nov 19, 2012 3:58 am
by DR
details please.
Re: Rising curve at the end of chromatogram
Posted: Mon Nov 19, 2012 6:49 pm
by Bigbear
Yes details.
If it is what I think, yes its normal. Most columns give a slightly rising baseline due to the temp. ramp. It looks worse if there is no major peaks to normalize on.
Re: Rising curve at the end of chromatogram
Posted: Tue Nov 20, 2012 7:32 am
by ito_miura
dear all,
so it's normal if there's no peak anymore in the end analysis the baseline is rising up?
Re: Rising curve at the end of chromatogram
Posted: Tue Nov 20, 2012 7:48 am
by Peter Apps
Let's try to get just one very basic piece of information - is this gas chromatography or liquid chromatography ?
Peter
Re: Rising curve at the end of chromatogram
Posted: Tue Nov 20, 2012 2:24 pm
by ito_miura
dear peter,
it is gas chromatrography with mass spectrometry and also pyrolysis...
Re: Rising curve at the end of chromatogram
Posted: Tue Nov 20, 2012 2:37 pm
by Peter Apps
Then the rising baseline is normal (presuming that you are doing temperature programming) and is usually due to column bleed and the elution of unresolved heavy compounds.
Peter
Re: Rising curve at the end of chromatogram
Posted: Wed Nov 21, 2012 2:58 am
by ito_miura
okey thank you if this is normally happen......
have a nice day....