How to prepare a DEAE-Sephadex A-25 (pyridine acetate form)?
Posted: Thu Nov 15, 2012 12:04 pm
I'm going to perform a Glucosinolate assay, I need to prepare a DEAE-Sephadex A-25 (40 mg) column (pyridine acetate form). I have pyridine, glacial acetic and the DEAE-Sephadex A-25 powder.
Can someone give me a protocol (preparation guide)? I have found two in google, but there are both little different.
Can someone give me a protocol (preparation guide)? I have found two in google, but there are both little different.