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Suitable Internal Standard for Folic Acid?

http://www.chromforum.org/viewtopic.php?t=21274

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Suitable Internal Standard for Folic Acid?

Posted: Mon Nov 12, 2012 1:47 pm
by shemesh199
Hello all , we are assaying folic acid by lc-ms-ms, are there any suitable internal standards to use. Ideally we are looking for something cheap, maybe a vitamin analog, methylparaben, folic acid 13c5? Anybody have any recommendations , and please list the vendor?

Thanks so much!

Re: Suitable Internal Standard for Folic Acid?

Posted: Fri Nov 23, 2012 8:18 am
by Alp
Stable isotope is always the best, but costs more.

If you are extracting the analyte then you want a similar compound as the IS so that it extracts the same way as the analyte and behaves the same in the ion source. I doubt methylparaben would be the best choice.
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