Chromatography Forum

Hi
We are about to receive a HUPLC method for transfer. The method was developed with a Waters UPLC. Currently we have an Agilent 1260 in our lab and we did some tests with the same column, mobile phase and flow as per the method and the instrument was capable of holding up the pressure. We haven't done any test with standards yet, but as soon it is available we will be doing so.
We are wondering if we should foresee any problem with the Agilent?

Is this an isocratic or gradient method? If it's a gradient method you'll have to account for the difference in delay volume between the 2 systems. If its isocratic there should be no problem.

Which injection mode was used on the Acquity, partial loop or partial loop with needle overfill?

Hi
We are about to receive a HUPLC method for transfer. The method was developed with a Waters UPLC. Currently we have an Agilent 1260 in our lab and we did some tests with the same column, mobile phase and flow as per the method and the instrument was capable of holding up the pressure. We haven't done any test with standards yet, but as soon it is available we will be doing so.
We are wondering if we should foresee any problem with the Agilent?

As mentioned you will need to take into account the gradient delay if it is gradient ...

The other POTENTIAL issues are:

1) Differences in dispersion pre and post column. The Acquity system and it's detectors are designed to be low dispersion (the new I-Class even more so than the original Acquity or the HClass). If the 1260 has the same or less dispersion than the Acquity the method was developed on, you will be OK. If not you will still be OK if the the peaks are not too close together But if it's vey close there could be issues.

3) Pressure: As time goes on LC column pressure tends to rise as it gets dirty. If you are close to the 1260 pressure limit with a new column, you may find you run into pressure issues sooner than you would like that you have to deal with.

- Karen