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Assay of D3 as per British pharmacopeia

http://www.chromforum.org/viewtopic.php?t=21015

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Assay of D3 as per British pharmacopeia

Posted: Mon Oct 15, 2012 1:32 pm
by ubhanu.pharma
Hi,
I have a problem in assay of Vitamin D3 in Normal phase with Pentenol and Hexan as Mobile phase, i need to know the Retention Time of Cis and trans for system suitability can any one help me

Re: Assay of D3 as per British pharmacopeia

Posted: Mon Oct 15, 2012 1:58 pm
by prepcolumn
1) Open the link below
2) Write Colecalciferol
3) Click chromatogram: available
4) You will see all peaks including trans impurity (vit D3: around 20 mins, trans isomer: around 11.5 mins)

https://extranet.edqm.eu/page7/page2/page2.html
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