RRF and calibration/quantitation calculations
Posted: Tue Sep 25, 2012 1:52 pm
Hello,
I am using Xcalibur for a GC/MS ITSD-quantitative analysis. I am not 100% sure of how does the software works. Does Xcalibur uses the RRF in order to quantify the native compounds according to the following well known equations?: From the Calibration: RRF=[Area (native)/Amount (native)*Amount (IS)/Area (IS)] and then in the sample: Amount (Native)= [Area (native)/RRF * Amount (IS)*Area (IS)]
And If so, where can I find the calculated RRF?.
Thanks very much
Regards
I am using Xcalibur for a GC/MS ITSD-quantitative analysis. I am not 100% sure of how does the software works. Does Xcalibur uses the RRF in order to quantify the native compounds according to the following well known equations?: From the Calibration: RRF=[Area (native)/Amount (native)*Amount (IS)/Area (IS)] and then in the sample: Amount (Native)= [Area (native)/RRF * Amount (IS)*Area (IS)]
And If so, where can I find the calculated RRF?.
Thanks very much
Regards