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- Posts: 6
- Joined: Fri Aug 10, 2012 9:20 pm
I'm trying to assay a drug in plasma extracts by a HPLC system equipped with DAD. The analyte has the maximum absorption at 341 nm, and no absorption around 450 nm. So I set the DAD condition on 341 nm for detection and 450 nm as the reference wavelenght. On the other hand, I'm using an isocratic elution, so I'm not sure if I basically need to use a reference wavelength or not. Can I expect improvement of the results, or a detrimental effect?