Page 1 of 1

UPLC grade water or HPLC grade water??

Posted: Fri Sep 21, 2012 12:11 am
by seamoro
Im running on UPLC for the last month and ive had serious pressure problems....i finally think its the water we are using. I found this article...... http://www.hplc.hu/PDFs/UHPLC_MobilePhasePrep.pdf. It suggests that even though i using hplc grade water filtered through 0.2um filter that this isnt good enough!! has anybody had experience of this??

Re: UPLC grade water or HPLC grade water??

Posted: Fri Sep 28, 2012 2:04 pm
by KRRISH8
I think problem with your column? Switch to new column.

Re: UPLC grade water or HPLC grade water??

Posted: Fri Sep 28, 2012 3:58 pm
by silmartras
Usually this filtration is enough. Check the prefilter of the column.