-
- Posts: 8
- Joined: Fri Feb 10, 2012 8:05 pm
Hello all. I am new pretty new to using hplc and had a general question about regenerating my column.
I've observed that my retention times slightly shift to higher values with the more injections I make. Once after about 9 injections had retention time had increased. And even when I increased temperature expecting the retention time to decrease, it was larger than the lower temperature. I looked up some normal-phase regeneration procedures. I only had one solvent for the regeneration procedure, but I used it anyways and saw a significant drop in the retention time.
My question is, how often should someone have to regenerate their column. After seeing this I was thinking of adding column regeneration to my method maybe every 6 injections. But I wasn't sure if that was overkill and if I should just reconsider my whole set up.
Thanks for any help.
I've observed that my retention times slightly shift to higher values with the more injections I make. Once after about 9 injections had retention time had increased. And even when I increased temperature expecting the retention time to decrease, it was larger than the lower temperature. I looked up some normal-phase regeneration procedures. I only had one solvent for the regeneration procedure, but I used it anyways and saw a significant drop in the retention time.
My question is, how often should someone have to regenerate their column. After seeing this I was thinking of adding column regeneration to my method maybe every 6 injections. But I wasn't sure if that was overkill and if I should just reconsider my whole set up.
Thanks for any help.
