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Yieng-Hau R. Han, Xue-Zhi Qin. Determination of alendronate sodium by ion chromatography with refractive index detection. J Chromatography A. 719(1996)345-352.
It has a published sensitivity of .4x10^-4mg/mL. We have run the experiment with the same column (Waters IC-Pak anion HR column) and a newer model of the RI detector along with the 6mM nitric acid as the mobile phase however we can only achieve .04mg/mL sensitivity. We are trying to find a method that's very sensitive as we're looking at small quantities of alendronate (which is also why we're trying to use methodologies that do not require complexation). I have contacted Merck (where the article was published from but the authors are no longer there and am waiting on other response from the company). Any suggestions for how to get increased sensitivity? We're using the temp, mobile phase, and concentration of mobile phase that they found to yield the best results at current.
Any suggestions for very sensitive method for alendronate measurement? We only have an RI and a UV detector (no conductivity or other specialized detectors). Thanks so much!!