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HPLC Carryover test

http://www.chromforum.org/viewtopic.php?t=20748

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HPLC Carryover test

Posted: Mon Sep 17, 2012 4:22 pm
by mk_3249
Hello Frnds

Recently we had an audit of EDQM. One of its auditor asked me a question about HPLC but I was unanswered.
The question was HPLC carryover test at the time of HPLC calibration.
Does anyone have any idea about how to perform this test?

Thank you.

Re: HPLC Carryover test

Posted: Mon Sep 17, 2012 7:27 pm
by tom jupille
The manufacturer should be able to provide detailed instructions, but in general you run a high-level standard of a single compound and follow with a diluent blank. The area ratios give you the carryover.
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