HPLC Carryover test

[mk_3249]

Hello Frnds

Recently we had an audit of EDQM. One of its auditor asked me a question about HPLC but I was unanswered.
The question was HPLC carryover test at the time of HPLC calibration.
Does anyone have any idea about how to perform this test?

Thank you.

[tom jupille]

The manufacturer should be able to provide detailed instructions, but in general you run a high-level standard of a single compound and follow with a diluent blank. The area ratios give you the carryover.