Chromatography Forum

We have a Waters 2475 Fluorescence detector and some time ago it showed 3 erros menssage, in sequence: "Calibration not found", "Method not found" e "PMT not calibrated". According to the technician the problem was a battery 3.0V Zeropower. This battery was replaced, and after replancing continued appearing message "PMT not calibrated". Then, following the instructions in the manual, it was performed calibration procedure using Milliq water. However, these procedures did not achieve success.

Could someone help me?

Thanks a lot.

Hi,

did you do the PMT calibration, normalization & calibration accuracy? when the battery is repalced then the following is completed and usually resolves the issues.

Regards,
Bull76

Hi Bull76,

Many thanks for your contribution!

I will try your suggestion and come back here to tell you the result.

By

We have a similar problem:
After replacing the battery, the Unit normalization and the PMT Calibration failed.
A Manually performed Unit normalization was stopped after 24 h. How long does this normalization normaly last? What can be a reason for this long duration?
Where can I manually start the PMT calibration?

Best regards
waldhorst

Hi,

Did you calibrate the PMT first, then the normalise units and then calibration. All these tests take less than half an hour to complete. Is there something else happening with the unit? Maybe it's power cycling on itself?
You'll need the access code to get to the PMT calibration page. Do you have a maintenance contract?

Regards,
Bull76.

Hi Bull76,
thanks for your fast reply - no we dont have a maintenance contract (no longer, expired years ago), but the Waters-Hotline was very helpfull.
I was able to calibrate the PMT, but now the unit normalization failed due to >3nm missmatch of the wavelength, although I used degassed UPLC-grade water. I cleaned the flow cell with 6N nitric acid (as suggested in the manual) and performed the normalization again, but I could not get better results. The Error still occures and I cant messure


Best
Waldhorst

Hi,

If the normalisation isn't passing after the flowcell has been cleaned and the system flushed with HPLC grade Water then you would need to get the optics serviced and that normal requires the unit been sent to a repair centre. When was the lamp replaced last and how long was the lamp turned on before you ran the normalisation? Can you swap out the flowcell for another to verify that the 6N nitrc acid has indeed cleaned the flowcell?

Regards,
Bull76.

Hi Bull76,
I replaced the lamp one month ago. But nevertheless, I also tried the calibration with an "old" lamp - same result.
The lamp was at least 48h powered on before I performed the calibration (or tried to...).
Unfortunally I do not have a flowcell for replacement available.

Tnx
waldhorst

Hi,

Do you have a different source of Water to use? Also what value are you getting for the Normalisation? It does sound like the optics are solarised and needs to be repaired. Only issue with that is the optics aren't a field replaceable parts. One can replace the optics bench but not the gratings. It would need to go to a repair centre for repair and this is actually more cost effective than a new optics bench.

Regards,
Bull76.