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How buffer work and used in Reverse phase chromatography ?

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How buffer work and used in Reverse phase chromatography ?

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Narendra
Hi Friends,

I want to know what is the roll of phosphate buffer or any salts like sodium aceate or citric acid etc. in reverse phase chromatography? Buffers helps in separation ? How to chose buffer for reverse phase chromatography ?

Thanks in advance.

Narendra - Mumbai

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HW Mueller
This has been discussed to death before, you should be able to find it via "search".
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