Advertisement

Correction Factors of related substances

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

5 posts Page 1 of 1
Hello everyone,
i am new in this forum so please excuse me if i make no sense!
to get straigth to the point, we have a dilemma as far as the calculation of correction factor of the related substances of Ranitidine HCl is concerned. Our final product is expressed as % Ranitidine (as base) but our working std is Ranitidine HCl. When calculating the % of the related substance we take into consideration the convertion of Ranitidine HCl to Ranitidine (base) but our problem is ...do we have to make this convertion when we calculate the correction factor of each related substance? I tried to make my point clear, i hope i did. If there is anyone that can help me please answer!

Thank you in advance
Calculate your standard concentration in terms of the base, then all is in terms of that including RS.
Thanks,
DR
Image
Thank you for your reply,
so, if i understood what you said, you mean to calculate my standard in terms of base in order to use it to the formula i use to calculate the correction factor of each RS getting a fixed number, which i will later use in the formula to calculate my %w/w of RS in my product -where i will have to calculate my standard in terms of base again (because my product's claim is expressed as base). Did i understand correcly? Does that mean that i do the conversion (Ranitidine HCl to base) twice or not?
Thank you for your time
You just do the conversion one time.

Calculate your ran.HCl concentration, then multuiply it by the ratio of
base formula weight / salt formula weight. This value is your standard concentration in the data system.
Thanks,
DR
Image
During the validation of RS i calculate the responce factor from the slope of Ranitidine HCl and the Relative Substance's slope. Do i make the conversion then at the concentration of Ranitidine HCl?
Thank you for your kind help!
5 posts Page 1 of 1

Who is online

In total there are 334 users online :: 1 registered, 0 hidden and 333 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Google [Bot] and 333 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry